Kit ɗin Gano Maganin Cutar COVID-19 Neutralizing Antibody
An yi niyyaAmfani
Wannan samfurin yana amfani da immunoassay na chromatographic na gefe don gano ingantacciyar gano ƙwayoyin rigakafin ƙwayoyin cuta a cikin jini, plasma, ko duka jini daga mutanen da suka karɓi alurar riga kafi ko waɗanda aka murmure daga COVIV-19.
TAKAITACCEN
Novel coronaviruses na cikin nau'in β.COVID-19 cuta ce mai saurin kamuwa da numfashi.Mutane gabaɗaya suna da sauƙi.A halin yanzu, marasa lafiya da suka kamu da cutar sankara ta coronavirus sune babban tushen kamuwa da cuta;masu dauke da cutar asymptomatic kuma na iya zama tushen kamuwa da cuta.Dangane da binciken cututtukan cututtuka na yanzu, lokacin shiryawa shine kwanaki 1 zuwa 14, galibi daga kwanaki 3 zuwa 7.Babban bayyanar cututtuka sun haɗa da zazzabi, gajiya da bushewar tari.Hakanan ana samun cunkoso na hanci, hanci, ciwon makogwaro, myalgia da gudawa a wasu lokuta.
KA'IDA
SARS-CoV-2 antibodies neutralizing antibodies su ne kariyar rigakafi da jikin mutum ke samarwa bayan alurar riga kafi ko kamuwa da cuta.Wannan kit ɗin yana amfani da mai karɓar ACE2 don haɗawa da gasa zuwa antigen S-RBD na hoto tare da kawar da ƙwayoyin rigakafi.Ya dace don gano sakamako na rigakafi bayan rigakafi ko kamuwa da cutar hoto.Tarin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da SARS-COV-2 S-RBD antigen da aka haɗa tare da gwal ɗin colloid da linzamin kwamfuta IgG-gold conjugates, 2) wani tsiri na nitrocellulose mai ɗauke da layin gwaji (T line) da layin sarrafawa (layin C).An riga an rufe layin T tare da mai karɓar ACE2.An riga an riga an lulluɓe layin C da goat anti linzamin kwamfuta IgG.Lokacin da isassun ƙarar samfurin aka bazu a cikin ramin lodin samfurin akan katin gwaji, samfurin yana ƙaura ta hanyar aikin capillary a fadin tsiri.Idan antibodies neutralizing suna nan a cikin samfurin, za su ɗaure zuwa S-RBD antigen akan gwal ɗin colloid, kuma su toshe wurin daurin masu karɓar ACE2.Saboda haka, tsiri zai sami raguwar ƙarfin launi a layin T ko ma rashin layin T.Idan samfurin bai ƙunshi ƙwayoyin rigakafi masu kashewa ba, S-RBD antigen akan gwal ɗin colloid zai ɗaure ga masu karɓar ACE2 tare da iyakar inganci.Saboda haka, tsiri zai sami ingantaccen launi a layin T.
KYAUTA
1. Katin Gwaji
2. Allurar Samfuran Jini
3. Mai zubar da jini
4. Buffer Bulb
AJIYA DA KWANTA
1. Ajiye fakitin samfurin a zazzabi 2-30°C ko 38-86°F, kuma kauce wa fallasa hasken rana.Kayan ya tsaya tsayin daka a cikin ranar karewa da aka buga akan lakabin.
2. Da zarar an buɗe jakar jakar alkama, za a yi amfani da katin gwajin da ke ciki cikin sa'a ɗaya.Dauke da dadewa ga yanayi mai zafi da ɗanɗano zai iya haifar da sakamako mara kyau.
3. An buga lambar kuri'a da ranar karewa akan lakabin.
GARGADI DA TSIRA
1. Karanta umarnin don amfani a hankali kafin amfani da wannan samfurin.
2. Wannan samfurin don amfanin kansa ne ta masu amfani da ƙwararru ko ƙwararru.
3. Wannan samfurin yana da amfani ga duka jini, jini, da samfuran plasma.Amfani da wasu nau'ikan samfurin na iya haifar da kuskure ko rashin inganci sakamakon gwajin.
4. Da fatan za a tabbatar cewa an ƙara adadin samfurin da ya dace don gwaji.Yawan samfurin da yawa ko kaɗan na iya haifar da sakamako mara kyau.
5. Idan layin gwaji ko layin sarrafawa ya fita daga taga gwajin, kar a yi amfani da katin gwajin.Sakamakon gwajin ba daidai ba ne kuma gwada samfurin tare da wani.
6. Wannan samfurin abu ne mai yuwuwa.KAR a sake sarrafa abubuwan da aka yi amfani da su.
7. Zubar da samfuran da aka yi amfani da su, samfurori, da sauran abubuwan da ake amfani da su azaman sharar lafiyar likita a ƙarƙashin ƙa'idodin da suka dace.
